Isolation and identification of L-asparaginase producing bacteria from soil and effluent of slaughterhouse in Isfahan

L-asparaginase is one of the most important treatments for acute lymphoblastic leukemia. The aim of this study was isolation and molecularly identification for L-asparaginase producer bacteria. In this study, samples were taken from the effluent and soil of a slaughterhouse in Isfahan. M۹ culture medium was used to screen the L-asparaginase producer bacteria. After screening, qualitative measurement of L-asparaginase was performed based on the color change in the media. The L-asparaginase producer bacteria were identified by biochemical and molecular methods. ۱۱۴ colonies were isolated from the samples. ۵ colonies were able to produce L-asparaginase. The halo diameter of L-asparaginase production in bacteria numbers SS۱۸, SS۱۹, SE۶۴, SE۱۱۲ and SE۱۱۴ were ۲۱, ۲۲, ۳۰, ۳۳ and ۵۵ mm, respectively. ۱۶SrRNA sequences showed that strain SS۱۸ was ۹۹% likely to belong to Stenotrophomonas maltophilia, strain SS۱۹ was ۹۹% likely to belong to Chryseobacterium indologenes, strain SE۶۴ was ۹۹% likely to belong to the genus Chryseobacterium, strain SE۱۱۲ was ۹۹% likely to belongs to Bacillus safensis species and strain number SE۱۱۴ probably ۱۰۰% belongs to Bacillus velezensis species.  Since, L-asparaginase from different bacteria has shown different anti-cancer effects, finding the L-asparaginase producer microorganisms is one of the ways that lead us to have an enzyme with ideal medical aspects. One of the best environments for finding these bacteria is slaughterhouse soil and effluent, which are high in protein and can therefore be a potential source of bacteria that produce L-asparaginase.