A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis

Introduction: Brucellosis or Malta fever is one of the most prevalent bacterial zoonosis which is transmitted to human being from animals. High fever, myalgia, and arthralgia of the large joints are the important symptoms. This study was aimed to evaluate the anti-brucellosis effect of ethanol extract of wild snapdragon on Brucella melitensis bacteria. Methods: This is an experimental in which after preparing the ethanol extract of wild Snapdragon (Scrophularia deserti), the anti-Brucellosis impressions of the extract on Brucella melitensis which is separated from goat’s abortive fetus were studied by serial dilution and disc diffusion method. Results: In disc diffusion test, the disc containing ۶۰ μg/ml extract the inhibition zone was ۹.۷mm after ۷۲, for streptomycin (۲۰ μg/disc) ۱۳.۳ mm and for streptomycin plus ۶۰ μg/disc extract discs it was ۲۱.۵ mm, after ۷۲ hours incubation at ۳۷ centigrade. After analyzing the data; the MIC for serial dilution test was ۵۲% (۵۷۶ μg/ml) for the first repetition and ۵۰% (۵۷۶ μg/ml) for the third repetition of the extract with OD۶۰۰=۱.۵۶. For standard medicine "streptomycin" with OD۶۰۰=۱.۶۷ it was ۵۲% (۳۶۰ μg/ml). Conclusion: Ethanol extract of wild Scrophularia deserti is effective on Brucellla melitensis and its consumption might be useful in these patients.