Mehrnoosh Sedighi
Hamid Nasri
Mahmoud Rafieian-kopaei
Seifollah Mortazaei

Introduction: Traditionally Achillea millefolium L. has been used to treat gastrointestinal disorders. In this study the hydroalcoholic extract of A. millefolium was evaluated on ileum contractions of Wistar rats. Materials and Methods: In this experimental study ۳۲ male Wistar rats were designated into ۴ equal groups, including: acetyl choline, KCl, extract plus acetyl choline and the group which received extract plus KCl. The isotonic contractions of ileum (induced by ۶۰ mM KCl or ۱ μM acetyl choline) in tyrode solution were recorded, under ۱ gr tension. Then, the effects of normal saline or extract (۱%) were evaluated. The percentage changes were calculated and compared in different groups using ANOWA and Tukey tests. Results: The mean of ileum contractions in acetyl choline and KCl groups were ۱۸.۸۳±۴.۹۱ and ۱۸.۳۱±۱۱.۱۲ (p=۰.۵). The percentage of contraction relieve in extract plus acetyl choline group was ۵۳.۱۶±۱۲.۰۶ and in extract plus KCl group was ۶۲.۹۶±۱۱.۰۸ (p=۰.۴). The contractions in extract groups were significantly less than acetyl choline or KCl groups (p<۰.۰۵). Conclusion: The results indicate that Achillea millefolium extract inhibits ileum contractions. Therefore, it might be used in patients to reduce ileum spasms.